Venkanna Narkuti, 1a1Andhra Pradesh State Forensic Science Laboratory, Red Hills, Hyderabad, India and Center for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, India
Ravi N. Vellanki, 2a2Center for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, India
Narasimha M. Oraganti, 33Andhra Pradesh State Forensic Science Laboratory, Red Hills, Hyderabad, India
Lakshmi N. Mangamoori, 44Center for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, India
aV. Narkuti and R.N. Vellanki contributed equally to this project.
Corresponding author: Prof. Lakshmi Narasu Mangamoori, Center for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad-500085, India Phone/Fax: +91-40-23156129,
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Citation Information. Clinical Chemistry and Laboratory Medicine. Volume 46, Issue 11, Pages 1586–1588, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2008.312, November 2008
Publication History: Received: 31/3/2008; accepted: 20/7/2008; published online: 17/11/2008
Abstract
Background: A paternity disagreement analyzed with 15 autosomal microsatellite markers indicated allele sharing between the mother, questioned child and the alleged father generating an inconclusive paternity result.
Design and methods: In total, 15 autosomal and 17 Y tandem repeat loci were analyzed using AmpFlSTR Identifiler, AmpFlSTR®Y-filer™ kits followed by six microsatellite markers on X chromosome in DNA extracted from peripheral blood samples of the mother, questioned child and alleged father.
Results: Microsatellite analysis examined with 15 autosomal short tandem repeats (STRs) indicated at least one allele sharing at 14 loci between the mother, questioned child and alleged father except for the TPOX locus where a paternal-child allele mismatch was observed. Y chromosome investigations using 17 repeat markers signified the case as non-paternity (exclusion). A complete match of the six X chromosome loci in the questioned child with the mother was observed.
Conclusions: Our investigations on inconclusive paternity due to atypical allele sharing in autosomal microsatellites were resolved with X- and Y-chromosome STR analyses confirming the case as non-paternity.
Clin Chem Lab Med 2008;46:1586–8.
Keywords allele sharing, exclusion, mismatch, paternity testing, short tandem repeats (STRs), X-STR, Y-STR
