Clinical Chemistry and Laboratory Medicine

Search

Advanced Search

Issue: Aug 2006

Clinical Chemistry and Laboratory Medicine
E-Table of Contents RSS Recommended Title Order

Bibliographic Data

Clinical Chemistry and Laboratory Medicine

ISSN (Print) 1434-6621

ISSN (Online) 1437-4331

DOI 10.1515/cclm.367

Article Tools

View Citations in

Related Articles

Search

Search this journal

Advanced Search

Most Downloaded Articles

  1. The entero-insular axis: implications for human metabolism

    Lakshminarayan R. Ranganath

  2. Haemolysis: an overview of the leading cause of unsuitable specimens in clinical laboratories

    Giuseppe Lippi, Norbert Blanckaert, Pierangelo Bonini, Sol Green, Steve Kitchen, Vladimir Palicka, Anne J. Vassault, Mario Plebani

  3. The laboratory in diabetes and prediabetes


  5. Author Index

>>View Top 20 Most Accessed Articles

Issue: Aug 2006

Volume 44, Number 8

< Previous Article

Next Article >

Download to Citation Mgr

Track Citations

Email

Add to Bookshelf

Track Citations

A robust liquid chromatography tandem mass spectrometry method for total plasma homocysteine determination in clinical practice

Véronique Ducros,

1. Département de Biologie Intégrée, Centre Hospitalier Universitaire, Grenoble, France

1
Hélène Belva-Besnet,

2. Applied Biosystems, Courtaboeuf, France

2
Bruno Casetta,

3. Applied Biosystems, Monza, Italy

3
Alain Favier

4. Département de Biologie Intégrée, Centre Hospitalier Universitaire, Grenoble, France

4
Corresponding author: Véronique Ducros, Département de Biologie Intégrée, Pavillon B, Centre Hospitalier Universitaire, BP 217, 38043 Grenoble cedex, France Phone: +33-476765484, Fax: +33-476765664,
Citation Information. Clinical Chemical Laboratory Medicine. Volume 44, Issue 8, Pages 987–990, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2006.178, 01/08/2006
Publication History: Received: //; accepted: //; published online: 31/07/2006

Abstract

Background: Total plasma homocysteine has emerged as an independent risk factor for vascular disease. To meet increasing requests by clinicians for this homocysteine determination, a rapid assay for a routine use has been developed.

Methods: A robust, stable, isotope-dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) method is described, including all the practical details and analytical performance results.

Results: The method allows homocysteine quantitation over a linear working range up to 100 μmol/L, with the limit of quantification estimated at a low value of 0.09 μmol/L. Total analytical imprecision is less than 4%. Accuracy was assessed by measuring the homocysteine concentration in a serum Standard Reference Material.

Conclusions: The method was demonstrated to be quick, reliable and cheap after 1 year of use on a time-shared instrument in our hospital unit.

Clin Chem Lab Med 2006;44:987–90.

Keywords homocysteine, HPLC, tandem mass spectrometry (MS/MS), routine practice

PDF (89.8 KB) PDF with Links (108.9 KB)

Permalink

http://dx.doi.org/10.1515/CCLM.2006.178

Citing Articles

Show All Citing Articles